The 1.7 kb EcoRI/HindIII fragment is single copy and was used to detect the BglII RFLP.
Vector
Construct size (kb): 8.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 4.5
Gene product: carboxypeptidase A1 (pancreatic)(carboxypeptidase A) [CPA]
Alleles: A1, A1, A2, A2
Insert Size (kb)
4.5
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
The insert contains the following sites separated by (kb)(approx): EcoRI- 1.5- BamHI- 1.2- HindIII 1.2- BglII- 0.5- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 4.4; HindIII--6.3, 2.5; PstI--6.5, 2.5; BamHI--6.8, 2.0; BamHI/HindIII--6.0, 1.6, 1.1, 0.1; EcoRI/HindIII--4.4, 2.7, 1.7.
Codominant segregation of the BglII RFLP was shown in 19 cystic fibrosis families (94 individuals) and 23 CEPH families (378 individuals).
The insert is the 3' flanking region of the human carboxypeptidase A (CPA) gene.
The 1.7 kb EcoRI/HindIII fragment is single copy and was used to detect the BglII RFLP.
