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ACY1-E
ACY1-E
規(guī)格:
貨期:
編號:B244623
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 ACY1-E
商品貨號 B244623
Designations ACY1-E
Species Homo sapiens, human
Depositors YE Miller
Vector
Construct size (kb): 4.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.384999990463257
Tissue: liver
Gene product: aminoacylase I(aminoacylase, aminoacylase 1) [ACY1]
Insert Size (kb) 1.385
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.4; PstI--3.1, 0.8, 0.15; HindIII--4.1; BamHI--4.1; SalI--4.1.
The insert contains the following restriction sites (nt from the 5' end): PstI--716, 875; MboI--1025.
The insert is oriented in the vector so as to generate a fusion protein.
Detects a genomic EcoRI fragment of 22 kb mapping to chromosome 3 and a genomic EcoRI fragment of 4.4 kb mapping to chromosome 18. The latter fragment is less intensely visualized in both EcoRI and KpnI digests.
Detects a major 0.9 kb mRNA and a minor 4.2 kb mRNA in human liver, fibroblasts, and a variety of cell lines. Also detects a 0.9 kb mRNA in the K1 Chinese hamster ovary cell line.
References

Miller YE, et al. Human aminoacylase-1: cloning, regional assignment to distal chromosome 3p21.1, and identification of a cross-hybridizing sequence on chromosome 18. Genomics 8: 149-154, 1990. PubMed: 1707030

York E Miller, personal communication

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