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B27M1
B27M1
規(guī)格:
貨期:
編號:B163978
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 B27M1
商品貨號 B163978
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/08A2 myeloma cells.
Clinical Data
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7).
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic
Comments
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7).
Spleen cells were fused with Sp2/08A2 myeloma cells.
The antibody is cytotoxic for HLA B27 and for HLA B7 at appropriate dilution.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
  • 0.05 mM 2-Mercaptoethanol

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
Isotype IgG2a
Name of Depositor F Grumet
Deposited As mouse (B cell); mouse (myeloma)
References

Grumet FC, et al. Monoclonal anti-HLA-B27 antibody (B27M1): production and lack of detectable typing difference between patients with ankylosing spondylitis, Reiter's syndrome, and normal controls. Lancet 2: 174-176, 1981. PubMed: 6166821

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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