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BL3.1
BL3.1
規(guī)格:
貨期:
編號(hào):B164036
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 BL3.1
商品貨號(hào) B164036
Organism Bos taurus, cow
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphosarcoma
Age 3 month old
Gender male
Strain Hereford
Applications
CRL-2306 can be used in BLV and retroviral studies and as a target for NK assays.
Storage Conditions liquid nitrogen vapor phase
Derivation

BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3.

The BL-3 cell line was isolated from a 3-month-old male Hereford calf.

BL-3 cells were exposed to gamma-irradiation and surviving cells were immunoselected for MHC class I antigen loss.
Genes Expressed
bovine leukemia virus (BLV)
Cellular Products
bovine leukemia virus (BLV)
Comments

BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant. The cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.

The cell line is positive for bovine leukemia virus (BLV) and actively produces BLV.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Name of Depositor JS Harms
Year of Origin 1990
References

Harms JS, Splitter GA. Impairment of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35: 1-8, 1992. PubMed: 1345904

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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