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CCD-1087Sk
CCD-1087Sk
規(guī)格:
貨期:
編號(hào):B164161
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CCD-1087Sk
商品貨號(hào) B164161
Organism Homo sapiens, human
Tissue skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 18 years
Gender female
Applications
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
Clinical Data
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
The patient had interstitial fibrosis and hyalinization consistent with macromastia.
female
Comments
The line was established from normal skin from the breast of a patient undergoing reduction mammoplasty.
The patient had interstitial fibrosis and hyalinization consistent with macromastia.
The cells senesce after approximately 43 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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