| 產(chǎn)品名稱 |
CCD-34Sk |
| 商品貨號(hào) |
B164208 |
| Organism |
Homo sapiens, human |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
2.5 weeks |
| Gender |
female |
| Ethnicity |
Black |
| Applications |
The line was derived from normal skin of a neonate that died of hemolytic anemia due to Rh incompatibility. A normal lung line derived form the same patient is available as ATCC CRL-1491. |
| Derivation |
The line was derived from normal skin of a neonate that died of hemolytic anemia due to Rh incompatibility. A normal lung line derived form the same patient is available as ATCC CRL-1491. |
| Clinical Data |
A normal lung line derived form the same patient is available as ATCC CRL-1491. The child was delivered at 34 weeks gestation. female Black 2.5 weeks |
| Comments |
The line was derived from normal skin of a neonate that died of hemolytic anemia due to Rh incompatibility. A normal lung line derived form the same patient is available as ATCC CRL-1491. The child was delivered at 34 weeks gestation. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| STR Profile |
Amelogenin: X CSF1PO: 11,13 D13S317: 9,13 D16S539: 8,13 D5S818: 12 D7S820: 10 THO1: 7,9 TPOX: 11 vWA: 15,16 |
