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CCRF-HSB-2 [CCRF HSB-2, HSB-2]
CCRF-HSB-2 [CCRF HSB-2, HSB-2]
規(guī)格:
貨期:
編號(hào):B164218
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CCRF-HSB-2 [CCRF HSB-2, HSB-2]
商品貨號(hào) B164218
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease acute lymphoblastic leukemia
Age 11.5 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters.
Clinical Data
11.5 years
Caucasian
male
Antigen Expression
HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%)
Tumorigenic Yes, in Syrian hamsters
Effects
Yes, in Syrian hamsters
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL.

A maximum of 2 to 3 x 106 viable cells/mL is obtainable.

Medium Renewal: Every 2 to 3 days

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18,19
Isoenzymes
G6PD, B
Name of Depositor GE Foley
Deposited As Homo sapiens
References

Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967.

Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716

Proc. Am. Assoc. Cancer Res. 8: 1, 1967.

Cross References

Nucleotide (GenBank) : NM_004429 Homo sapiens ephrin-B1 (EFNB1), mRNA.

Nucleotide (GenBank) : U09303 Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds.

Nucleotide (GenBank) : NM_004429 Homo sapiens ephrin-B1 (EFNB1), mRNA.

Nucleotide (GenBank) : U09303 Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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