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CON.1
CON.1
規(guī)格:
貨期:
編號:B164287
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CON.1
商品貨號 B164287
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Animals were immunized with a peptide corresponding to residues 23 to 44 in bovine clathrin LCa and LCb (a region that is conserved in all mammalian clathrin light chains).
The antibody is useful for western blotting, immunofluorescence and immunoprecipitation applications involving clathrin light chains of mammals.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human clathrin light chain
Cellular Products
immunoglobulin; monoclonal antibody; against human clathrin light chain
Comments
Animals were immunized with a peptide corresponding to residues 23 to 44 in bovine clathrin LCa and LCb (a region that is conserved in all mammalian clathrin light chains).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful for western blotting, immunofluorescence and immunoprecipitation applications involving clathrin light chains of mammals.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be established by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 105 and 106 viable cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor FM Brodsky
Deposited As mouse (B cell); mouse (myeloma)
References

Nathke IS, et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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