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CW13.20-3B3 (clone of BCL1)
CW13.20-3B3 (clone of BCL1)
規(guī)格:
貨期:
編號:B164307
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CW13.20-3B3 (clone of BCL1)
商品貨號 B164307
Organism Mus musculus, mouse
Tissue peripheral blood
Cell Type B lymphocyte
Product Format frozen
Morphology clusters of lymphoblast-like cells and single attached cells with a dendritic-like appearance
Culture Properties Mixed: adherent and suspended
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease leukemia
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Antigen Expression
H-2d
Genes Expressed
immunoglobulin
Comments
This line does not produce IgM unless stimulated with B Cell Differentiation Factor (BCDF, interleukin-6, interleukin 6, IL-6), however some lots of fetal bovine serum contain levels of endotoxin which may induce a background level of IgM secretion. In order for cells to function optimally in the BCDF assay using IgM secretion, it is essential to pretest fetal bovine serum (FBS) and select lots that are low in endotoxins and do not induce high background levels of IgM secretion. Background secretion should be less than 400 ng/mL.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.05 mM 2-mercaptoethanol

Subculturing
The cells loosely adhere to the substrate, and subcultures are prepared when the culture is 75% to 90% confluent by striking the flask sharply 3 to 4 times against the palm of the hand. Free floating cells, which accumulate with time in culture, are viable and can be used to initiate new cultures.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Isotype IgM
Name of Depositor K Brooks, E Vitetta
Deposited As Mus musculus
References

Brooks K, et al. Lymphokine-induced IgM secretion by clones of neoplastic B cells. Nature 302: 825-826, 1983. PubMed: 6601774

Cross References

Nucleotide (GenBank) : U08185 Mus musculus BALB/c zinc-finger protein Blimp-1 mRNA, complete cds.

Nucleotide (GenBank) : NM_007548 Mus musculus PR domain containing 1, with ZNF domain (Prdm1), mRNA.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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