精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Cy34.1.2
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Cy34.1.2
Cy34.1.2
規(guī)格:
貨期:
編號(hào):B164308
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Cy34.1.2
商品貨號(hào) B164308
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with spleen cells from B10.D2 mice.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against Lyb 8.2 (mouse B cell antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against Lyb 8.2 (mouse B cell antigen)
Comments

The antibody defines an antigen on mouse B cells that is expressed on pre B cells and mature B cells.

The antibody is NOT cytotoxic and precipitates molecules with apparent molecular weights between 95000 and 105000 daltons.

The Lyb 8.2 antigen expression is controlled by a locus on chromosome 7 that is closely linked to the Gpi-1 and RP-2 loci.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain culture density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C 
Atmosphere: 5% CO2

Isotype IgG1
Name of Depositor FW Symington
Deposited As mouse (B cell); mouse (myeloma)
References

Sprent J, et al. Lyb-8.2: a new B cell antigen defined and characterized with a monoclonal antibody. Immunogenetics 16: 381-391, 1982. PubMed: 6984010

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
枣强县| 江达县| 天等县| 温泉县| 铁力市| 蓬莱市| 奉化市| 巴林右旗| 邛崃市| 江陵县| 白银市| 玉山县| 宁蒗| 余干县| 长武县| 英德市| 当阳市| 萍乡市| 马关县| 蒙城县| 瓮安县| 灌云县| 瑞安市| 金湖县| 建始县| 定结县| 合作市| 大关县| 新绛县| 无棣县| 鹿泉市| 吉安市| 南宁市| 习水县| 韶山市| 肇源县| 锡林郭勒盟| 玛纳斯县| 乐东| 鱼台县| 金塔县|