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DATK32
DATK32
規(guī)格:
貨期:
編號:B164332
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DATK32
商品貨號 B164332
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Lymphoma
Applications
The antibody reacts with the mouse lymphocyte mucosal homing receptor.
It is specific for mouse alpha 4, beta 7 heterodimers, and can induce alpha 4, beta 7 dependent lymphocyte aggregation.
DATK32 is useful in flow cytometry methods using sensitive second stage reagents, but is ineffective in immunohistological staining.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine alpha 4, beta 7 integrin
Cellular Products
immunoglobulin; monoclonal antibody; against murine alpha 4, beta 7 integrin
Comments
Animals were immunized with cells of the TK1 murine T cell lymphoma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the mouse lymphocyte mucosal homing receptor.
It is specific for mouse alpha 4, beta 7 heterodimers, and can induce alpha 4, beta 7 dependent lymphocyte aggregation.
The antibody inhibits alpha 4, beta 7 mediated adhesion activities including binding to MAdCAM-1, and alpha 4, beta 7 dependent VCAM-1 and fibronectin binding.
DATK32 is useful in flow cytometry methods using sensitive second stage reagents, but is ineffective in immunohistological staining.
Complete Growth Medium RPMI 1640 medium with 4.5 g/L glucose,15 mM HEPES, 2 mM L-glutamine, 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG2a
Name of Depositor EC Butcher, DP Andrew
Deposited As rat (B cell); mouse (myeloma)
References

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506

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