精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > DSL-6B/C2
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
DSL-6B/C2
DSL-6B/C2
規(guī)格:
貨期:
編號(hào):B164374
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DSL-6B/C2
商品貨號(hào) B164374
Organism Rattus norvegicus, rat
Tissue pancreas
Cell Type azaserine induced
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease pancreatic carcinoma
Gender male
Strain Lewis
Applications
DSL-6B/C2 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6B/C2 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
Storage Conditions liquid nitrogen vapor phase
Derivation
DSL-6B/C2 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6B/C2 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
Clinical Data
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
male
Genes Expressed
mucin
Cellular Products
mucin
Tumorigenic Yes
Effects
Yes, in Lewis rats the cells produce solid tumors and partially cystic tumors composed with a mixed phenotype of squamous, mucinous and glandular areas
Comments
DSL-6B/C2 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma.
The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat (DSL-101-79) that was given azaserine intraperitoneally.
The cultured DSL-6B/C2 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture.
The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting.
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin-0.53 mM ) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:3 to 1:4
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor OS Pettengill, D Longnecker
Deposited As Rattus sp.
Year of Origin 1986
References

Durfee T, et al. Derivation of ductlike cell lines from a transplantable acinar cell carcinoma of the rat pancreas. Am. J. Pathol. 143: 292-303, 1993. PubMed: 8391218

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
兰考县| 龙胜| 新竹市| 白银市| 玛沁县| 彭山县| 南投市| 苍溪县| 阜康市| 长丰县| 黄梅县| 历史| 中江县| 安义县| 农安县| 武强县| 凤庆县| 微山县| 邵阳县| 巴南区| 临颍县| 大冶市| 庆城县| 友谊县| 金塔县| 丽水市| 大石桥市| 大关县| 隆尧县| 广灵县| 乐安县| 灵丘县| 南部县| 依兰县| 通海县| 固始县| 永仁县| 定边县| 新田县| 沁源县| 承德市|