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DU1-29
DU1-29
規(guī)格:
貨期:
編號:B164379
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DU1-29
商品貨號 B164379
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the sheep L-selectin molecule expressed on populations of T cells, B cells, neutrophils, medullary thymocytes and monocytes.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against ovine and bovine L-selectin (CD62L)
Cellular Products
immunoglobulin; monoclonal antibody; against ovine and bovine L-selectin (CD62L)
Comments
Animals were immunized with CD4-, CD8- sheep thymocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the sheep L-selectin molecule expressed on populations of T cells, B cells, neutrophils, medullary thymocytes and monocytes.
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor CR Mackay
Deposited As mouse (B cell); mouse (myeloma)
References

Mackay CR, et al. Tissue-specific migration pathways by phenotypically distinct subpopulations of memory T cells. Eur. J. Immunol. 22: 887-895, 1992. PubMed: 1372559

梅經(jīng)理 17280875617 1438578920
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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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