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Dubca
Dubca
規(guī)格:
貨期:
編號(hào):B164381
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Dubca
商品貨號(hào) B164381
Organism Camelus dromedarius, camel
Tissue skin
Cell Type SV40 transformed
Product Format frozen
Morphology fibroblast
Culture Properties monlayer
Biosafety Level 2   [Cells contain papovavirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Autopsy
Age 2 months gestation
Gender female
Applications
SV40 large T-antigen is detected by Western blotting.
The cell line is susceptible to infection with viruses of several different families.
The cells may be used for virus diagnosis and vaccine production.
Derivation
The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.
Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.
Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.
Clinical Data
female
Virus Resistance
parapox virus; canine adenovirus type 1 (CAV); Aleutian disease virus (ADV); influenza virus (A equi 1); ECBO virus, equine arteritis virus (EAV)
Comments
The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.
Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.
SV40 large T-antigen is detected by Western blotting.
The cell line is susceptible to infection with viruses of several different families.
Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.
While some isolates of bovine viral diarrhea virus (BVDV) and border disease virus (BDV) could not be grown in Dubca cells other isolates could be propagated to a limited extent (only a few cells expressed viral antigen).
The cells may be used for virus diagnosis and vaccine production.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor OR Kaaden
Deposited As Camelus dromedarius
References

Klopries M, et al. Characterization of the camel skin cell line Dubca. Br. Vet. J. 151: 555-565, 1995. PubMed: 8556315

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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