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E.H. IV (Elaine IV)
E.H. IV (Elaine IV)
規(guī)格:
貨期:
編號:B164386
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 E.H. IV (Elaine IV)
商品貨號 B164386
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease infectious mononucleosis
Age 20 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
Epstein-Barr virus (EBV)
Cellular Products
Epstein-Barr virus (EBV)
Virus Resistance
partially resistant to poliovirus and vesicular stomatitis viruses
Comments
The cells carry and produce Epstein-Barr virus (EBV).
EBNA positive.
Biological Safety CLASS II.
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium at 4 to 5 x 105 viable cells/mL. A maximum of 1 to 2 x 106 cells/mL is obtainable.

Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions Temperature: 37°C
Atmosphere: 5% CO2
Isoenzymes
G6PD, B
Name of Depositor W Henle
Deposited As Homo sapiens
References

Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134

Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. JAMA 203: 205-209, 1968. PubMed: 4864269

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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