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E5BB3IIA2
E5BB3IIA2
規(guī)格:
貨期:
編號(hào):B164388
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 E5BB3IIA2
商品貨號(hào) B164388
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody induces histamine release from human basophils.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified IgE myeloma protein.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human IgE
Cellular Products
immunoglobulin; monoclonal antibody; against human IgE
Comments
Animals were immunized with purified IgE myeloma protein.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody induces histamine release from human basophils.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine
adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, and 1.0 mM
sodium pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a; kappa light chain
Name of Depositor RP Siraganian
Deposited As mouse (B cell); mouse (myeloma)
References

Hook WA, et al. Detection of different antigenic sites on human IgE using monoclonal antibodies. Fed. Proc. 42: 713, 1983.

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沈經(jīng)理 19548299266 2662369050
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