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EGC/PK060399egfr
EGC/PK060399egfr
規(guī)格:
貨期:
編號:B164395
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EGC/PK060399egfr
商品貨號 B164395
Organism Rattus norvegicus, rat
Tissue jejunum
Cell Type enteroglial
Product Format frozen
Morphology glial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult adult
Gender male
Strain Sprague-Dawley
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Genes Expressed
glial fibrillary acidic protein (GFAP); S-100 protein and vimentin
Cellular Products
glial fibrillary acidic protein (GFAP); S-100 protein and vimentin
Comments
The cells display robust glial fibrillary acidic protein (GFAP), S-100 and vimentin immunoreactivities, but no Thy-1.1, desmin, smooth muscle alpha-actin or C3 complement receptor immunoreactivity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor A Ruhl
Deposited As rat
Year of Origin March 6, 1999
References

Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat. Neurogastroenterol. Motil. 13: 95-106, 2001. PubMed: 11169131

The EGC/PK060399egfr enteroglial cell line was established on March 6, 1999 from adult rat myenteric plexus from the jejunum. Purified primaries were transfected with supernants from the virus producing cell line GPE-egfr-neu. GPE-egfr-neu cells contain the chimeric gene egfr/neu and the neomycin-resistance gene neo under the control of the long-terminal repeats of the mouse Moloney leukaemia virus. The egfr/neu gene codes for the extracellular domain of the human EGF receptor and the intracellular tyrosine kinase domain of the proto-oncogene c-neu.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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