精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > EOMA-GFP
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
EOMA-GFP
EOMA-GFP
規(guī)格:
貨期:
編號(hào):B164414
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EOMA-GFP
商品貨號(hào) B164414
Organism Mus musculus, mouse
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2 Cells contain SV40 and HSV viral sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease hemangioendothelioma
Age adult
Applications
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP).
The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse.
Storage Conditions liquid nitrogen vapor phase
Derivation
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1).
The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse.
Comments
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1).
The vector contains cytomegalovirus (CMV), SV40 and Herpes simplex virus (HSV) viral DNA sequences and the neomycin resistance gene.
The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse.
For additional details see EOMA (ATCC CRL-2586).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • 0.2 mg/ml G -418
  • fetal bovine serum to a final concentration of 10%
  • Subculturing
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37.0°C
    Name of Depositor R Auerbach
    Deposited As mouse
    Year of Origin 1998
    References

    Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185

    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
    玛沁县| 巴东县| 荔波县| 阆中市| 曲麻莱县| 石阡县| 富蕴县| 华宁县| 常德市| 万荣县| 宝山区| 贞丰县| 东海县| 于都县| 衡阳县| 阳城县| 青铜峡市| 武清区| 乌兰浩特市| 桂林市| 嘉黎县| 安乡县| 呼伦贝尔市| 长沙市| 南昌县| 秦安县| 故城县| 盐城市| 沂水县| 容城县| 陕西省| 饶平县| 云阳县| 乐陵市| 阿尔山市| 波密县| 洛南县| 武乡县| 鄂托克前旗| 伊宁市| 太仆寺旗|