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F3B6
F3B6
規(guī)格:
貨期:
編號:B164428
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 F3B6
商品貨號 B164428
Organism human (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The cells are resistant to 6-thioguanine.
This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.
Comments
Fusion of human peripheral blood B cell with NS-1 myeloma cells.
The cells are resistant to 6-thioguanine.
This line is suitable for fusing to human B cells for the production of human monoclonal antibodies.
Complete Growth Medium
Culture Medium: HL-1 medium, 99%; fetal bovine serum, 1%. Iscove's modified
Dulbecco's medium with 10% fetal bovine serum has been used as an alternative
to HL-1.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 1 X 10 exp6 cells/ml.
Cryopreservation
HL-1 medium, 82.5%, human serum albumin, 10%; DMSO, 7.5%
Name of Depositor Cetus Corp.
Deposited As human (B cell); mouse (myeloma)
U.S. Patent Number
References

Larrick JW, Raubitschek AA. Pseudomonas aeruginosa exotoxin A antibodies, their preparation and use. US Patent 4,677,070 dated Jun 30 1987

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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