精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > FeT-1C
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
FeT-1C
FeT-1C
規(guī)格:
貨期:
編號:B164452
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 FeT-1C
商品貨號 B164452
Organism Felis catus, cat
Tissue peripheral blood mononuclear cells (PBMC); T lymphocyte
Cell Type T lymphocyte
Product Format frozen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The cell line was derived from a specific pathogen free (SPF) cat [U.S. Pat. 5,846,825].
Virus Susceptibility Feline immunodeficiency virus
Comments

The FeT-1C cell line (ATCC CRL-11968) is IL-2 dependent, whereas the FeT-J cell line (ATCC CRL-11967) is IL-2 independent [U.S. Pat. 5,846,825] This feline T-cell line is susceptible to infection by feline immunodeficiency virus (FIV) [U.S. Pat. 5,846,825].


Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 Units/ml human recombinant IL-2 and 10% heat-inactivated fetal bovine serum
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor University of Florida, Gainesville
Deposited As cat
U.S. Patent Number
References

Yamamoto JK. Feline derived T-cell lines for producing FIV. US Patent 5,846,825 dated Dec 8 1998

Hohdatsu T, et al. Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence. J. Gen. Virol. 77: 93-100, 1996. PubMed: 8558132

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
株洲市| 沧源| 天水市| 商丘市| 昔阳县| 库伦旗| 汤原县| 宜君县| 林周县| 家居| 巫溪县| 措美县| 诏安县| 汾阳市| 周宁县| 鸡西市| 兴义市| 滕州市| 革吉县| 东安县| 洪江市| 宜黄县| 新河县| 宜兰县| 漠河县| 崇文区| 黄石市| 且末县| 巴林左旗| 牙克石市| 佛冈县| 包头市| 天长市| 平果县| 昭平县| 永康市| 怀仁县| 清新县| 常州市| 同仁县| 化州市|