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FHCR-1-2624/FH6/FHOT-1-3019 [FH6]
FHCR-1-2624/FH6/FHOT-1-3019 [FH6]
規(guī)格:
貨期:
編號(hào):B164455
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) FHCR-1-2624/FH6/FHOT-1-3019 [FH6]
商品貨號(hào) B164455
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Adenocarcinoma,Cancer
Applications
The antibody reacts with the 6B ganglioside and not with other structurally related gangliosides.
The FH6 antibody reacts with a variety of human cancer cells, but does not react with normal human tissues.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified 6B ganglioside mixed with Salmonella minnesota.
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against 6B ganglioside, Lex, (tumor [adenocarcinoma] associated fucoganglioside)
Cellular Products
immunoglobulin; monoclonal antibody; against 6B ganglioside, Lex, (tumor [adenocarcinoma] associated fucoganglioside)
Comments
Animals were immunized with purified 6B ganglioside mixed with Salmonella minnesota.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with the 6B ganglioside and not with other structurally related gangliosides.
The 6B ganglioside is absent in normal colon tissue, but accumulates in adenocarcinomas of the colon.
The FH6 antibody reacts with a variety of human cancer cells, but does not react with normal human tissues.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol, 88%; fetal bovine serum, 12%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor Fred Hutchinson Cancer Res. Cntr.
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Hakomori S. Hybridoma antibody (FH6) defining a human cancer-associated difucoganglioside. US Patent 4,904,596 dated Feb 27 1990

Kim YS, et al. Lex and Ley antigen expression in human pancreatic cancer. Cancer Res. 48: 475-482, 1988. PubMed: 3335015

Nakasaki H, et al. Mosaicism in the expression of tumor-associated carbohydrate antigens in human colonic and gastric cancers. Cancer Res. 49: 3662-3669, 1989. PubMed: 2659167

Rosenman SJ, et al. Murine embryonal carcinoma cell-surface sialyl LeX is present on a novel glycoprotein and on high-molecular-weight lactosaminoglycan. Exp. Cell Res. 180: 326-340, 1989. PubMed: 2464500

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胡經(jīng)理 13345964880 2438244627
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沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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