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FIB504.64
FIB504.64
規(guī)格:
貨期:
編號(hào):B164462
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 FIB504.64
商品貨號(hào) B164462
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Lymphoma
Applications
The antibody reacts with human and mouse beta 7 integrin.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine beta 7 integrin
Cellular Products
immunoglobulin; monoclonal antibody; against murine beta 7 integrin
Comments
Animals were immunized with cells of the TK1 murine T cell lymphoma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with human and mouse beta 7 integrin.
It blocks lymphocyte binding to MAdCAM-1, and alpha 4, beta 7 integrin binding to VCAM-1 and fibronectin.
The antibody can inhibit alpha 4, beta 7 dependent binding of lymphocytes to intestinal epithelia.
Complete Growth Medium RPMI 1640 medium with 4.5 g/L glucose,15 mM HEPES, 2 mM L-glutamine, 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor EC Butcher, DP Andrew
Deposited As rat (B cell); mouse (myeloma)
References

Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506

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