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G2a.5
G2a.5
規(guī)格:
貨期:
編號(hào):B164479
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) G2a.5
商品貨號(hào) B164479
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
Cellular Products
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
Comments
Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.
Spleen cells were fused with NS-1 myeloma cells.
This line is a switch variant of Genox 3.53 (ATCC HB-103).
An IgG2b switch variant of Genox 3.53 also exists (G2b.2, ATCC HB-109).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640 with HT (containing: hypoxanthine, 10(-4) M;
thymidine, 1.6 x 10(-5) M), 90%; fetal bovine serum, 10%.
Cells do not do well if hypoxanthine and thymidine are omitted.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor P Parham
Deposited As mouse (B cell); mouse (myeloma)
References

Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068

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