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G-7
G-7
規(guī)格:
貨期:
編號:B164485
品牌:Mingzhoubio

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產(chǎn)品名稱 G-7
商品貨號 B164485
Organism Mus musculus, mouse
Tissue skeletal muscle
Cell Type myoblast myoblast
Product Format frozen
Morphology myoblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age fetus fetus
Strain Swiss
Applications
They are slightly responsive to acetylcholine.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 40
Tumorigenic Yes
Effects
Yes,
Comments
When confluent, the cells fuse to form multinucleated myotubes.
They are slightly responsive to acetylcholine.
The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Subculturing The cells should be subcultured before they become confluent.
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Add 2.0 to 3.0 mL of 0.25% Trypsin-0.53mM EDTA solution to flask and  observe cells under an inverted microscope until cell   layer is  dispersed (usually within 2 to 5 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  4. Add appropriate aliquots of the cell suspension to new  Bovine Collagen type l coated flasks. (0.03 mg/mL).
  5. Incubate cultures at 37°C. Myotubes form at confluency. Differentiation is improved by reducing the concentration of both sera to 2% each.

Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 3 to 4 days

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time 17 to 19 hrs
Name of Depositor J Peacock
Deposited As Mus musculus
References

Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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