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GAD-1
GAD-1
規(guī)格:
貨期:
編號(hào):B164490
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 GAD-1
商品貨號(hào) B164490
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with GAD in cells from chickens, mice, rats, rabbits, cats and macaque monkeys.
The enzyme GAD is present in and is a marker for neurons that use gamma amino butyric acid as a neurotransmitter.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glutamic acid decarboxylase (GAD)
Cellular Products
immunoglobulin; monoclonal antibody; against glutamic acid decarboxylase (GAD)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with GAD.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with GAD in cells from chickens, mice, rats, rabbits, cats and macaque monkeys.
The enzyme GAD is present in and is a marker for neurons that use gamma amino butyric acid as a neurotransmitter.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor DI Gottlieb
Deposited As mouse (B cell); mouse (myeloma)
References

Gottlieb DI, et al. Monoclonal antibodies to glutamic acid decarboxylase. Proc. Natl. Acad. Sci. USA 83: 8808-8812, 1986. PubMed: 2430303

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李經(jīng)理 13626845108 972239479
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