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GAP 8.3
GAP 8.3
規(guī)格:
貨期:
編號(hào):B164493
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 GAP 8.3
商品貨號(hào) B164493
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human leukocyte (monocytes, lymphocytes, granulocytes); against CD45
Cellular Products
immunoglobulin; monoclonal antibody; against human leukocyte (monocytes, lymphocytes, granulocytes); against CD45
Comments
Mice were immunized with MOLT-4 cells a human T cell line (MOLT-4, ATCC CRL-1582).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes a 200000 dalton antigen analogous to the mouse T200 antigen and the rat common leukocyte antigen (it does not react with erythrocytes).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype IgG2a; kappa light chain
Name of Depositor AE Berger
Deposited As mouse (B cell); mouse (myeloma)
References

Berger AE, et al. A human leukocyte antigen identified by a monoclonal antibody. Hum. Immunol. 3: 231-245, 1981. PubMed: 6975769

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李經(jīng)理 13626845108 972239479
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