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Genox 3.53
Genox 3.53
規(guī)格:
貨期:
編號:B164502
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Genox 3.53
商品貨號 B164502
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the DQw1 (DC10) determinant of the HLA D region.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against DQw1 (DC1, HLA DR)
Cellular Products
immunoglobulin; monoclonal antibody; against DQw1 (DC1, HLA DR)
Comments
Animals were immunized with glycoproteins from the Bristol 8 human B lymphoblastoid cell line.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with the DQw1 (DC10) determinant of the HLA D region.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor P Parham
Deposited As mouse (B cell); mouse (myeloma)
References

Brodsky FM, et al. Monoclonal antibodies to HLA -- DRw determinants. Tissue Antigens 16: 30-48, 1980. PubMed: 6162228

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