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GL7
GL7
規(guī)格:
貨期:
編號(hào):B164508
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 GL7
商品貨號(hào) B164508
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse lymphocytes
Cellular Products
immunoglobulin; monoclonal antibody; against mouse lymphocytes
Comments
Animals were immunized with LPS activated DBA/2J mouse B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells.
The GL7 antigen is expressed on up to 70% of activated B cells and on high proportions of ConA activated CD4 + and CD8 + T cells.
Unstimulated B and T cells are infrequently GL7 positive.
The antigen appears to be a lymphocyte activation antigen that is different from those previously identified.
Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate,
1% nonessential amino acids and 50 ?M 2-mercaptoethanol, 90%; fetal bovine
serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgM
Name of Depositor G Lazlo, KS Hathcock
Deposited As rat (B cell); mouse (myeloma)
References

Laszlo G, et al. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252-5262, 1993. PubMed: 8515058

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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