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HFN 7.1

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編號(hào):B164603

品牌:Mingzhoubio

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產(chǎn)品名稱(chēng)	HFN 7.1
商品貨號(hào)	B164603
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue	spleen
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	Tested and found negative for ectromelia virus (mousepox).
Storage Conditions	liquid nitrogen vapor phase
Derivation	Mice were immunized with purified human fibronectin. Spleen cells were fused with P3X63Ag8 myeloma cells.
Antigen Expression	H-2d
Genes Expressed	immunoglobulin; monoclonal antibody; against human fibronectin,H-2d
Cellular Products	immunoglobulin; monoclonal antibody; against human fibronectin
Comments	The antibody does not cross-react with other fibronectins tested (26 species including 15 primates). Mice were immunized with purified human fibronectin. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). Formerly ATCC HB-39.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG1
Name of Depositor	RC Schoen, KL Bentley, RJ Klebe
Deposited As	mouse (B cell); mouse (myeloma)
References	Schoen RC, et al. Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

序號(hào)	文章標(biāo)題	操作
1	ATCC細(xì)胞運(yùn)輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
4	購(gòu)買(mǎi)須知	查看詳情
5	訂購(gòu)流程	查看詳情

梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479

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