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Jensen Sarcoma
Jensen Sarcoma
規(guī)格:
貨期:
編號:B164890
品牌:Mingzhoubio

標準菌株
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DNA
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產(chǎn)品名稱 Jensen Sarcoma
商品貨號 B164890
Organism Rattus norvegicus, rat
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Virus Resistance
poliovirus 2
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Every day (when possible)
Place culture in cold (+4C) for 60 minutes, shake flask to suspend cells, centrifuge, resuspend in fresh medium and dispense into new flasks.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor MK Patterson
Deposited As Rattus sp.
References

Proc. Soc. Exp. Biol. Med. 100: 115, 1959.

. . Z. Krebsforsch 7: 45, 1908.

Patterson MK Jr., Orr G. L-asparagine biosynthesis by nutritional variants of the Jensen sarcoma. Biochem. Biophys. Res. Commun. 26: 228-233, 1967. PubMed: 6067662

Patterson MK Jr., et al. Studies on the asparagine requirement of the Jensen sarcoma and the derivation of its nutritional variant. Cancer Res. 29: 296-300, 1969. PubMed: 5765412

Patterson MK Jr., et al. Studies on the aspartic acid "sparing effect" on the nutritional requirement of L-asparagine for tumors in vitro. Proc. Soc. Exp. Biol. Med. 131: 131-134, 1969. PubMed: 5770086

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