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<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
規(guī)格:
貨期:
編號:B232473
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Saccharomyces cerevisiae Meyen ex E.C. Hansen
商品貨號 B232473
Deposited As Saccharomyces cerevisiae Hansen, teleomorph
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations MW3271-10C pms1-delta
Alternate State Candida robusta Diddens et Lodder
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Type Strain no
Genotype MATa pms1-delta leu2-3 leu2-112 lys2-20 his4-519 ade8-deltaKpn ura3-52 hom3-10 thr1 lys1-1 ade2 arg4-deltaSst1 RefWilliamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926, RefKramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974, (Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.)
Preceptrol&reg; no
Mating Type a
Ploidy haploid
Comments
Meiosis-sporulation: postmeiotic segregation increase (CL2)
Medium ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation.
Subcultivation
Protocol: The "quick" method for checking pms1 is to print on canavanine media: papillation (mutability) will be increased. The same result is obtained by printing on met- media and treating with UV; increased papillation will occur due to enhanced mutability at the hom3-10 locus in the presence of the pms1 mutation.
Name of Depositor YGSC
Special Collection Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--W. Kramer and K. Lusnak
References

Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926

Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974

Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.

Williamson MS, et al. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2. Genetics 110: 609-646, 1985. PubMed: 3896926

Kramer W, et al. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB. J. Bacteriol. 171: 5339-5346, 1989. PubMed: 2676974

Isogenic with MW3271-10C (ATCC 208348) except for the pms locus.

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