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ISG54genomic [lambda-3]
ISG54genomic [lambda-3]
規(guī)格:
貨期:
編號:B233946
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 ISG54genomic [lambda-3]
商品貨號 B233946
Designations ISG54genomic [lambda-3]
Species Homo sapiens, human
Vector
Construct size (kb): 47.0
Insert
DNA: genomic
Insert lengths(kb): 15.0
Tissue: liver
Gene product: interferon-induced protein 54(interferon, alpha-inducible protein (54kD, ISG-54K, IFI-54K), interferon-stimulated gene, 54KDa protein) [G10P2]
Insert Size (kb) 15.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Insert contains the following sites separated by (bp)(approx): EcoRI- 50- HindIII- 2970- HindIII- 3630- EcoRI- 124- EcoRI- 375- EcoRI- 120- HindIII- 440- EcoRI- 909- EcoRI- 770- HindIII- 1430- EcoRI...(not the complete insert).
Restriction digests of the clone give the following sizes (kb): XbaI--19.5, 15.0, 6.4 (doublet); HindIII--21.0, 7.4, 6.0, 5.4 (doublet), 1.0; SalI--uncut; EcoRI--19.5, 10.7, 6.7 (doublet), 3.4; BamHI--28.0, 7.2, 5.8, 4.2, 1.6, 0.45.
Region from nt -104 to -90 acts as an interferon-inducible enhancer.
Contains the RNA initiation site, exons 1 and 2 plus intervening sequence, and polyA signal of human ISG-54K.
The protein predicted from the DNA sequence has a possible limited homology at the amino acid level with murine Mx protein.
References

Levy D, et al. Interferon-stimulated transcription: isolation of an inducible gene and identification of its regulatory region. Proc. Natl. Acad. Sci. USA 83: 8929-8933, 1986. PubMed: 3466167

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