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M31A
M31A
規(guī)格:
貨期:
編號:B234093
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 M31A
商品貨號 B234093
Designations M31A
Species Homo sapiens, human
Vector
Construct size (kb): 51.70000076293945
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: tumor necrosis factor, beta (lymphotoxin)(tumor necrosis factor, beta, lymphotoxin) [TNFA]
Insert Size (kb) 40.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--18.0, 9.6, 7.8, 4.8, 2.0, 1.7, 1.4, 0.7; KpnI--32.0, 11.4, 6.7, 1.6; SalI--38.3, 8.4, 5.1; PstI--5.2, 5.1, 3.5,
3.5, 3.4, 3.2, 3.0, 2.8, 2.8, 2.3, 2.1, 2.0, 1.6, 1.5, 1.4, 1.4, 1.1, 0.9, 0.7, 0.7, 0.65, 0.65.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid contains coding sequences for both TNFalpha and TNFbeta; fragment containing both can be excised by KpnI/BamHI digest (approx. 7 kb).
References

Spies T, et al. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc. Natl. Acad. Sci. USA 83: 8699-8702, 1986. PubMed: 3464978

Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413

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