精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pJS97
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pJS97
pJS97
規(guī)格:
貨期:
編號(hào):B234346
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pJS97
商品貨號(hào) B234346
Designations pJS97
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 7.3000001907348630
Vector: pJS97 (YAC)
Promoters: Promoter T7 (phi10)
Marker(s):URA3,ampR,SUP11
Construct size (kb): 7.300000190734863
Features: marker(s): ampR, URA3, SUP11
promoter: T7 (phi10)
replicon: pMB1, ARSH4
centromere: CEN4
Applications
YL-type (YAC) shuttle vector
shuttle vector
vector for constructing genomic libraries
vector for other uses
vector permitting RNA synthesis in vitro
Comments
Restriction digests of the clone give the following sizes (kb): NotI--7.4; BglII--7.4; SalI--7.4; EcoRI--7.4; SacI--7.4.
pJS97 (ATCC 77153) and pJS98 (ATCC 77154) are used together to construct artificial chromosomes. Each plasmid is digested with ClaI and the cloning enzyme.
To recover both ends of a YAC insert as plasmid, DNA of a YAC-containing strain is digested to completion with BamHI, SphI or SacI, diluted, ligated, and transformed into E. coli (to ampicillin resistance).
The insert of a resultant plasmid (which includes the T7 promoter) extends from an end of the YAC insert to the first site of the enzyme used for digestion.
YL-type shuttle vector useful for construction of genomic libraries. Contains promoters for in vitro RNA synthesis and permits recovery of end fragments as plasmids in E. coli.
The order of the major features in this plasmid is: ClaI/MCS/EcoRI - T7 promoter - ARSH4 - CEN4 - URA3 - SUP11 - pMB1 ori - bla - SacI - BamHI - SphI - TEL.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Shero JH, et al. Yeast artificial chromosome vectors for efficient clone manipulation and mapping. Genomics 10: 505-508, 1991. PubMed: 2071158

Related Products
component of:ATCC 77191
Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
隆尧县| 湘西| 隆尧县| 丰原市| 婺源县| 昭苏县| 乌拉特前旗| 建始县| 东乡县| 锡林郭勒盟| 迭部县| 云阳县| 库伦旗| 四川省| 松滋市| 休宁县| 奉贤区| 红桥区| 榆林市| 淮北市| 四平市| 察雅县| 忻州市| 东乡县| 赤城县| 桦南县| 化隆| 永顺县| 宁河县| 张家港市| 鄂尔多斯市| 侯马市| 灌阳县| 绥棱县| 泰州市| 桐庐县| 普定县| 延川县| 二连浩特市| 锦屏县| 类乌齐县|