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Vaccinia virus
Vaccinia virus
規(guī)格:
貨期:
編號:B234389
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 Vaccinia virus
商品貨號 B234389
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain Elstree (Lister Vaccine)
Biosafety Level 2 Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses (Biosafety in Microbiological and Biomedical Laboratories, 5th edition. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2009.)

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
Derived from bacterially contaminated ATCC® VR-862™. Treated with multiple freeze thaws and filtrations (0.45 µm filters twice and 0.2 µm filter once) to remove bacterial contaminants.
Effect on Host
In cell culture, cells appear swollen and syncytial, with eventual rounding and sloughing of cells; forms plaques.
On chorio-allantoic membrane (CAM) virus forms pocks.
Recommended Host
Vero cells (ATCC® CCL-81™?)
Growth Conditions

Recommendations for Infection:  Plant cells 24 hours in advance and infect when cultures are 70-80% confluent.   Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI of about 0.1.  Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding VGM.

Incubation: Incubate infected Vero cell culture for 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. Incubate for 3 days on CAM.

Name of Depositor JH Nakano and ATCC
Special Collection Orthopoxvirus
Chain of Custody
Deposited as Vaccinia virus, Orthopoxvirus
Source ATCC® VR-1549™ is derived from bacterially contaminated ATCC® VR-862™. Treated with multiple freeze thaws and filtrations (0.45 µm filters twice and 0.2 µm filter once) to remove bacterial contaminants. ATCC® VR-862™ was originally derived from sheep.
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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