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pGBM6
pGBM6
規(guī)格:
貨期:
編號(hào):B234416
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGBM6
商品貨號(hào) B234416
Designations pGBM6
Depositors J Geiselmann
Biosafety Level 1
Vector Information
Size (kb): 4.4279999732971190
Vector: pGBM6 (plasmid)
Promoters: Promoter lac
Construction: pSC101, pUC18 MCS
Marker(s):spcR,strR
Construct size (kb): 4.427999973297119
Features: insert detection: lacZ'
marker(s): strR/spcR
promoter: lac
replicon: pSC101
MCS: HindIII...EcoRI
Applications
The vector can be used whenever it is necessary to maintain two plasmids within the same cell.
The vector expresses the alpha amino-terminal fragment of the lacZ gene product and display alpha-complementation in appropriate hosts.
Comments
Restriction digests of the clone give the following sizes (kb): PvuI--3.4, 1.05; BglI-- 2.4, 1.1, 0.9; HindIII-- 4.4.
The vector can be used whenever it is necessary to maintain two plasmids within the same cell.
The vector expresses the alpha amino-terminal fragment of the lacZ gene product and display alpha-complementation in appropriate hosts.
The vector was constructed by inserting 241/678 fragment of pUC18 into pGBM1 (ATCC 87497).
Media ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin
Growth Conditions
Temperature: 37.0°C
References

Manen D, et al. A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322. Gene 186: 197-200, 1997. PubMed: 9074496

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