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U6.2
U6.2
規(guī)格:
貨期:
編號:B234573
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 U6.2
商品貨號 B234573
Designations U6.2
Species Homo sapiens, human
Depositors N Dahl
Applications
This probe is useful for carrier and prenatal diagnosis in fragile-X families.
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.0
Gene product: DNA Segment, single copy [DXS304]
Alleles: A1, A2
Insert Size (kb) 1.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.8, 2.3; EcoRI--5.4; EcoRI/HindIII--4.4, 1.0; HindIII--5.4.
Co-segregated with the fragile-X phenotype in 29 informative meioses in 9 fragile-X families.
This probe is useful for carrier and prenatal diagnosis in fragile-X families.
The TaqI insertion/deletion polymorphism is also detected by the following enzymes (the most frequent size listed first in parenthesis): StuI (8.7/9.0), BglI (5.3/5.9), BclI (2.9/3.3), MspI (4.5/2.2), PstI (6.6/7.2).
References

Dahl N, et al. A polymorphic locus at Xq27-28 detected by the probe U6.2 (DXS304). Nucleic Acids Res. 17: 2884, 1989. PubMed: 2566159

Dahl N, et al. Isolation of a DNA probe of potential use for diagnosis of the fragile-X syndrome. Hum. Genet. 82: 216-218, 1989. PubMed: 2567272

Dahl N, et al. Linkage analysis of families with fragile-X mental retardation, using a novel RFLP marker (DXS 304). Am. J. Hum. Genet. 45: 304-309, 1989. PubMed: 2569270

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Suthers GK, et al. Physical mapping of new DNA probes near the fragile X mutation (FRAXA) by using a panel of cell lines. Am. J. Hum. Genet. 47: 187-195, 1990. PubMed: 2378346

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