精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > pGEM-7Zf(+)/LIC-F
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pGEM-7Zf(+)/LIC-F
pGEM-7Zf(+)/LIC-F
規(guī)格:
貨期:
編號(hào):B234586
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGEM-7Zf(+)/LIC-F
商品貨號(hào) B234586
Designations pGEM-7Zf(+)/LIC-F
Depositors RS Haun
Other IDs

Nucleotide (GenBank) : U25267 Ligation-independent cloning vector pBluescript II KS(+)/LIC, complete sequence.

Biosafety Level 1
Vector Information
Size (kb): 3.0329999923706060
Vector: pGEM-7Zf(+)/LIC-F (phagemid)
Promoters: Promoter T7
Construction: pGEM-7zf(+)
Marker(s):ampR
Construct size (kb): 3.032999992370606
Features: insert detection: lacZ
marker(s): ampR
promoter: SP6
promoter: T7
promoter: lac
replicon: f1
replicon: pMB1
MCS: ApaI...NsiI
Applications
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--2.95; EcoRI--2.95; BamHI--2.95.
Preparation of the vector for cloning includes linearization with NarI, gel purification of the linearized vector, and treatment with T4 DNA polymerase in the presence of dATP.
The target sequence can be amplified using sequence specific primers modified at the 5' end to contain an additional 13 nt complementary to the vector sequence.
The forward primer should contain 5'-CTGGTTCCGGCGA-3' followed by 12-15 nt target specific sequence. The reverse primer should contain 5'-CTCGCTCCGGCGA-3' followed by 12-15 nt target specific sequence.
Following amplification, the amplified sequence should also be gel purified and treated with T4 DNA polymerase in the presence of dTTP. Annealing of the vector and amplification product forms a duplex molecule that can be used directly for transformation.
Sequences amplified using these primers are also compatible with the pBluescript II KS(+)/LIC and pGEM-7Zf(+)/LIC-R vectors (ATCC 87047 and 87049).
Differs from pGEM-7Zf(+)/LIC-R (ATCC 87049) only in the orientation of complementary ends generated at the cloning site.
Ligation-independent cloning vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Haun RS, et al. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13: 515-518, 1992. PubMed: 1362067

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
玛沁县| 苍山县| 伊宁市| 土默特右旗| 沧州市| 青阳县| 鄂托克前旗| 景宁| 勐海县| 凌云县| 镇江市| 台北县| 常宁市| 樟树市| 广南县| 漳平市| 沂源县| 汶川县| 玉山县| 南召县| 乳山市| 内黄县| 青海省| 泾阳县| 衡阳市| 改则县| 北川| 融水| 闸北区| 湘阴县| 长宁县| 阳信县| 卫辉市| 卢湾区| 宜丰县| 武山县| 酒泉市| 凌云县| 怀仁县| 五原县| 兴仁县|