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pGEM-7Zf(+)/LIC-F
pGEM-7Zf(+)/LIC-F
規(guī)格:
貨期:
編號(hào):B234586
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGEM-7Zf(+)/LIC-F
商品貨號(hào) B234586
Designations pGEM-7Zf(+)/LIC-F
Depositors RS Haun
Other IDs

Nucleotide (GenBank) : U25267 Ligation-independent cloning vector pBluescript II KS(+)/LIC, complete sequence.

Biosafety Level 1
Vector Information
Size (kb): 3.0329999923706060
Vector: pGEM-7Zf(+)/LIC-F (phagemid)
Promoters: Promoter T7
Construction: pGEM-7zf(+)
Marker(s):ampR
Construct size (kb): 3.032999992370606
Features: insert detection: lacZ
marker(s): ampR
promoter: SP6
promoter: T7
promoter: lac
replicon: f1
replicon: pMB1
MCS: ApaI...NsiI
Applications
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--2.95; EcoRI--2.95; BamHI--2.95.
Preparation of the vector for cloning includes linearization with NarI, gel purification of the linearized vector, and treatment with T4 DNA polymerase in the presence of dATP.
The target sequence can be amplified using sequence specific primers modified at the 5' end to contain an additional 13 nt complementary to the vector sequence.
The forward primer should contain 5'-CTGGTTCCGGCGA-3' followed by 12-15 nt target specific sequence. The reverse primer should contain 5'-CTCGCTCCGGCGA-3' followed by 12-15 nt target specific sequence.
Following amplification, the amplified sequence should also be gel purified and treated with T4 DNA polymerase in the presence of dTTP. Annealing of the vector and amplification product forms a duplex molecule that can be used directly for transformation.
Sequences amplified using these primers are also compatible with the pBluescript II KS(+)/LIC and pGEM-7Zf(+)/LIC-R vectors (ATCC 87047 and 87049).
Differs from pGEM-7Zf(+)/LIC-R (ATCC 87049) only in the orientation of complementary ends generated at the cloning site.
Ligation-independent cloning vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Haun RS, et al. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13: 515-518, 1992. PubMed: 1362067

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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