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Naegleria fowleri Carter
Naegleria fowleri Carter
規(guī)格:
貨期:
編號:B234729
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Naegleria fowleri Carter
商品貨號 B234729
Strain Designations PA-90
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
domestic water supply, Port Augusta, Australia, 1972
Product Format frozen
Type Strain no
Comments
use of plastic ampoules for freeze preservation
Medium ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Growth Conditions
Temperature: 35.0°C
Duration: axenic
Cryopreservation
1.? Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.

2.? Adjust the concentration of cells to 2.0 x 106/ml.? If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.

3.?? Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows:? Add the required volume of DMSO to a glass screw-capped test tube and place on ice.? Allow the DMSO to solidify.? Add the required volume of refrigerated ATCC medium 1034.? Dissolve the DMSO by inverting several times.? If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.

4.? Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 60 min.

5.? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.? Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge the ampules into liquid nitrogen.

7.? The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.

8.?? To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.

9.?? Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 5.0 ml of fresh ATCC medium 1034.

10.????????? Incubate the tube on a 15° horizontal at 35°C with the cap screwed on tightly.

Name of Depositor JA Jamieson
Year of Origin 1972
References

Anderson K, Jamieson A. Agglutination test for the investigation of the genus Naegleria. Pathology 4: 273-278, 1972. PubMed: 4641057

Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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