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phPMCA2-1 [lambdat10.1-3.0]
phPMCA2-1 [lambdat10.1-3.0]
規(guī)格:
貨期:
編號:B234814
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 phPMCA2-1 [lambdat10.1-3.0]
商品貨號 B234814
Designations phPMCA2-1 [lambdat10.1-3.0]
GenBank Number

X63575

Species Homo sapiens, human
Depositors EE Strehler
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: teratoma NTera2D cell line
Gene product: ATPase, Ca++ transporting, plasma membrane 2 [PMCA2]
Alleles: A1, A2, A1, A2
Insert Size (kb) 3.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.7; PstI--2.8, 2.05, 0.55, 0.35; XhoI--5.7; BamHI--4.5, 1.1; HindIII--5.7.
The insert includes the following restriction sites (kb from the 5' end): PstI--0.21, 0.72, 2.68; XhoI--1.83; BamHI--1.86, 2.91; SacI--2.72, 2.82.
Extends from nt 410 to the EcoRI site at nt 3584, beginning at the codon for aa 114. Does not include 93 bp in the 5' coding region identified in some clones.
References

Heim R, et al. Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region. Eur. J. Biochem. 205: 333-340, 1992. PubMed: 1313367

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