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pEM303 [pHUMDUT2]
pEM303 [pHUMDUT2]
規(guī)格:
貨期:
編號:B235149
品牌:Mingzhoubio

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產(chǎn)品名稱 pEM303 [pHUMDUT2]
商品貨號 B235149
Designations pEM303 [pHUMDUT2]
GenBank Number

M89913

Species Homo sapiens, human
Depositors EM McIntosh
Applications
in another host, produces protein dUTP pyrophosphatase
Vector
Construct size (kb): 3.700000047683716
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DUT*
Genomic copy number: multiple
Gene name: dUTP pyrophosphatase (dUTPase)
Contains complete coding sequence?: Y
Chromosome: 15; Localization: 15, cR
Type of DNA: cDNA
Insert end: Modification: EcoRI adapters
Insert end: Modification: EcoRI adapters
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M89913
Insert lengths(kb): 0.800000011920929
Gene product: dUTP pyrophosphatase [DUT*]
Target Gene: dUTP pyrophosphatase
Insert Size (kb) 0.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.7; SmaI--3.7; SphI--3.7; EcoRI--2.9, 0.8; PstI--3.7.
The insert contains the following restriction sites (nt from the 5' end): SphI--43; SmaI--91, XmnI--653.
Contains the complete coding sequence, and corresponds to nt 43-837 of the database record.
The sense strand is packaged.
pEM303 (ATCC 79036/7) and pEM304 (ATCC 79038/9) differ only in the orientation of the insert.
References

McIntosh EM, et al. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc. Natl. Acad. Sci. USA 89: 8020-8024, 1992. PubMed: 1325640

Evan M McIntosh, personal communication

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