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pCG19
pCG19
規(guī)格:
貨期:
編號:B235521
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCG19
商品貨號 B235521
Designations pCG19
Depositors M Keller
Biosafety Level 1
Vector Information
Size (kb): 5.0479998588562000
Vector: pCG19 (plasmid)
Promoters: Promoter lambda PR
Construction: lambda pR promoter and cI857 repressor cloned into pCRII, ligated to pSC101/par and tetR from pLG339
Marker(s):tetR
Construct size (kb): 5.0479998588562
Features: marker(s): tetR
other: par locus
promoter: lambda PR
replicon: pSC101
repressor gene: cI857
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI - 5.0; XhoI - 5.0; PstI - 5.0.
Low copy number expression vector for overexpression of cloned bacterial* genes in E. coli from the lambda pR promoter, induced by temperature or pH shift, (the latter according to literature only).
Media ATCC® Medium 6025: LB Medium with 12 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References

Gotting C, et al. Versitile low-copy number plasmids for temperature-inducible overexpression of bacterial genes in Escherichia coli. BioTechniques 24: 362-366, 1998. PubMed: 9526640

Shipped frozen
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